of Science with First Class Honours in Molecular Biology
University of Tasmania, Hobart, Australia 1992 - 1995
immunohistochemical and PCR techniques I investigated the expression and
distribution of the metallothionein proteins in the
nasal mucosa of normal and double knock-out mice and their response to chemical
insult (Skabo 1997).
Laboratory Manager / Senior Research Assistant / PhD Sept 2011 – present
Clem Jones Centre for Regenerative Medicine, HSM, Bond University, Queensland
· Carried out a novel hESC to RPE adherent differentiation with >90% conversion.
· Designed and carried out tissue culture based bioengineering experiments using primary cells, MSCs, RPEs, endometrial, as well as neural stem cells, iPSC and hES cells. Analysed these experiments using SEM, TEM, Confocal microscopy as well as qPCR, IHC and FACS.
· Co-developed the CJRC’s 2013-2015 Research Plan, successfully approved for $1.8M and a project Extension Proposal which was the basis for a further $1.2M over 2016-2018.
· Co-authored three published, peer-reviewed papers (Warnke 2012, Alamein 2013a,b).
· Contributed significantly to and managed submission process for five NHMRC Project Grants (2013, 2015), an NHMRC LIEF grant (2012), a Smart State grant (QLD, 2012), BUPA Foundation (2014) and a Macular Degeneration Foundation grant (2015).
· Commenced a PhD titled “Alternative stem cell sources for retinal tissue engineering.”
Laboratory Manager / Senior Research Assistant March 2008 – May 2011
Stem Cell Engineering Group, AIBN, University of Queensland
· Designed and constructed transcription-factor containing lentiviral vectors, produced high titre lentivirus by transfection and used the viruses to transduce and successfully reprogram a variety of human primary cell lines into induced pluripotent stem-cells (iPS).
· Transfected and selected stable clones of various reporter-gene driven human adult and stem-cell lines. Maintained and differentiated these lines into lineage specific cell types. Characterised these lines using qPCR and immunohistochemistry.
· Produced and expressed iPS reprogramming factor proteins.
· Equipped and installed a full, working, molecular biology and tissue culture laboratory on time and under budget.
· Managed OH&S, OGTR and training compliance requirements; carried out ordering, financial management and maintenance for a 12 person/ $3M p.a. laboratory.
Senior Technical Officer July 2002 - July 2007
QABC, Department of Primary Industries and Fisheries, University of Queensland
· Created a novel method to produce an inverted repeat library in custom designed plasmid vectors for the production of siRNA from arbitrary genomic material.
· Developed a novel sequencing method to sequence several novel circular-DNA viruses including BBTV (Sharman 2007), PSMV and CSMV.
· Used fluorescent-SSR fingerprinting to identify commercial species (Holton, 2007)
· Undertook many new roles including Radiation Safety Officer, Workplace Health and Safety Representative, Fire Warden and accredited First Aid and Resuscitation Officer.
· Shared Laboratory Manager duties with two colleagues including workplace inductions, budgeting for and procuring large capital items, ordering of consumables, organising and overseeing repairs and writing and maintaining documentation.
Supervised visiting scientists, trained students
and colleagues including training for national accreditation (NATA) purposes.
Research Technician October 2000 - June 2002
QABC, SPIRT Research Grant, DPI and Botany Department, University of Queensland
· Developed novel methods for plastid transformation in agricultural crops.
· Transformed cultured tobacco with novel targeting-peptide+GFP fusion constructs and used fluorescent confocal microscopy to record the successful subcellular localisations.
· Learned a number of molecular biology techniques including, biolistic and agrobacterial plant transformations, plant and animal tissue culture, DNA vector construction, protein expression and purification.
· Completed a successful OGTR application for our project's GMO research.
· Qualified for a Queensland Government radiation "Use License".
Research Assistant March 1999 – June 2000
Royal Hobart Hospital, Molecular Endocrinology Laboratory, MEN1 Research Group
· Developed a genetic screening protocol for a disease-causing single nucleotide polymorphism, found in a large Tasmanian family, based on PCR and sequencing.
· Successfully prepared the Royal Hobart Hospital’s Molecular Endocrine Laboratory for NATA accreditation of genetic screening and diagnostic protocols and facilities.
· Initiated published work on a mRNA fusion-gene expression study in paraffin embedded tumour tissues involving technically difficult extraction and RT-PCR procedures.
Research Assistant January 1996 - January 1998
Cooperative Research Centre for Sustainable Production Forestry, Uni. of Tasmania
· Performed statistical analysis, co-wrote and published a population genetics study of Eucalyptus globulus utilising RAPD markers.
· Designed, budgeted for, carried out and published an industry group genetic QC project.
microsatellite markers from my own E.globulus clonal library.
· Highly proficient with office and scientific software as well as experience with laboratory hardware and software installation, use and maintenance.
· Vacation Scholarship Researcher, Summer 1995, CSIRO Marine Laboratories Hobart
· Summer Research Scholarship Summer 1994 UTAS Chemistry Department
· Divisional election campaign coordinator (‘04, ‘07) and BCC ward candidate (’04,’08).
Captained several sporting teams, 1997 Tasmanian
foil fencing champion.
Assistant Professor Dr Qin Liu
Alamein MA, Liu Q, Stephens S, Skabo S, Warnke F, Bourke R, Heiner P, Warnke PH. (2013a) Nanospiderwebs: artificial 3D extracellular matrix from nanofibers by novel clinical grade electrospinning for stem cell delivery. Adv Healthc Mater. 2013 May;2(5):702-17. Epub 2012 Nov 1.
Alamein MA, Stephens S, Liu Q, Skabo S, Warnke PH. (2013b) Mass production of nanofibrous extracellular matrix with controlled 3D morphology for large-scale soft tissue regeneration. Tissue Eng Part C Methods. 2013 Jun;19(6):458-72. Epub 2012 Dec 12.
Warnke PH, Alamein M, Skabo S, Stephens S, Bourke R, Heiner P, Liu Q (2012) Primordium of an artificial Bruch's membrane made of nanofibers for engineering of retinal pigment epithelium cell monolayers. Acta Biomater. 2013 Dec;9(12):9414-22. Epub 2013 Aug 2.
M Sharman, JE Thomas, S Skabo, TA Holton (2007) Abacá bunchy top virus, a new member of the genus Babuvirus (family Nanoviridae) Archives of Virology, 153 (1). pp. 135-147.
Conference proceeding: TA Holton, SJ Skabo, KF Lowe, K Sinclair (2007) Genetic fingerprinting of natural kikuyu populations in Australia TROPICAL GRASSLANDS 41;(3), 236-237
Acknowledgement: Gnanasambandam, A, Ian G. Polkinghorne and Robert G. Birch (2007) Heterologous signals allow efficient targeting of a nuclear-encoded fusion protein to plastids and endoplasmic reticulum in diverse plant species Plant Biotechnology Journal 5 (2): 290-296
Conference proceeding: "ABACA BUNCHY TOP VIRUS – A NEW BABUVIRUS?" M Sharman, JE Thomas, S Skabo and TA Holton (2004) The 6th Australasian Plant Virology Workshop
Burgess, JR, Skabo, SJ, McArdle, K, Tucker, P (2003) Temporal trends and clinical correlates for the RET/PTC1 mutation in papillary thyroid carcinoma. ANZ Journal of Surgery. 73 (1-2): 31-35
S. Skabo, R. E. Vaillancourt and B. M. Potts (1998) Fine-scale genetic structure of Eucalyptus globulus ssp. globulus forest. Australian Journal of Botany. 46 (5,6): 583-594
Vaillancourt, R.E., Skabo, S. and Gore, P.L. (1998) Fingerprinting for quality control in breeding and deployment. Australian Forestry. 61 (3): 207-210
Skabo, SJ, Chuah, MI and West, AK (1997) Metallothioneins 1 and 2 are expressed in the olfactory mucosa of mice in untreated animals and during the regeneration of the epithelial layer. Biochem Biophys Res Commun 232(1):136-42